Developement of a LAMP (Loop-mediated isothermal amplication) assay for detection of Fasciola spp.

Abstract

Fasciola spp. have two species, Fasciola gigantica and Fasciola hepatica, which cause disease mainly in herbivores such as buffaloes, cows, sheep, etc. and cause disease in humans. According to the World Health Organization (WHO), the disease is currently circulating in 75 countries and territories around the globe. WHO considers the disease a health issue that needs attention in public health programs and has been ranked as an important position in health strategy and policy by many countries. Therefore, developing a fast and accurate technique to detect Fasciola infection is very important and necessary. In this study, the authors developed the LAMP technique to detect Fasciola spp. from different kinds of sample such as feces, tissue samples, etc. The advantages of this method included high specificity, fast detection time, and simple equipment use. Primer set was designed for founding on ITS2 gene. A positive reaction was visualised with the naked eye, using the color indicator, malachite green.