Study on culture conditions for extracellular alginate lyase production by Bacillus velezensis AlgSm1.

Abstract

Alginate lyase is an enzyme that degrades alginate to create bioactive oligoalginate for application in medicine, industry, agriculture… In this study, cultural conditions of marine bacteria, Bacillus velezensis AlgSm1 have been investigated for producing alginate lyase with high content and activity. The nutritional components such as carbon source, nitrogen source, initial pH value, and cultural time were investigated. The results showed that the cultural conditions of B. velezensis AlgSm1 to biosynthesize alginate lyase with high activity were 5 mg/ml alginate, 0.8 mg/ml yeast extract; pH 5.5 and 18-hours fermentation at 28-30°C.