Research on technical production of Trichoderma preparations for control of yellow leaf - root rot on citrus trees

Abstract

It is necessary to study conditions biomass multiplication and production of microbial organic fertilizers from Trichoderma for citrus trees, to help improve soil and prevent Fusarium sp., Phytophthora sp. causes yellow leaf - root rot. This study used two very high resistance strains of Trichoderma fungi for culture, namely T.haz.22. QH and T.asp.11.TT. Cultures of Trichoderma in liquid medium with the composition of extract from 150 g bean sprouts + 30 g sugar + 1 g urea + 1 g KH2P04 + 0.5 g MgSO4 achieved the highest biomass of 4.35±0.48 g/l after 1 day. Solid fermentation medium using simmered whole-grain rice substrate and 10% fungal juice were suitable for fast growth and spores 8.82x109 CFU/g and obtained fungal biomass after 5-6 days. Producing micro-organic fertilizers using 100% manure or 50% bagasse + 50% manure with 6-8 kg of Trichoderma and obtained faecal biomass after 60 days of incubation.