Development of expression vector with the Pgrac100 promoter induced by IPTG and integrated gfp+ gene into Bacillus subtilis genome at lacA locus

  • Thi Bich Phuong Chu, Duc Hoang Nguyen, Thi Phuong Trang Phan
  • Thi Bich Phuong Chu
  • Thi Phuong Trang Phan*
Keywords: Bacillus subtilis, integrative, Isopropulβ-D- 1-thiogalactopyranoside-inducible vector, lacA locus, Pgrac100.

Abstract

Bacillus subtilis is a prospective gram - positive bacterium that offers an opportunity for the production of recombinant proteins in the pharmaceutical,
agricultural, industrial, and food industries. The goal of this study was to develop an integrative inducible expression system by incorporating the Pgrac100
promoter into the lacA locus of the B. subtilis genome...

Tác giả

Thi Bich Phuong Chu, Duc Hoang Nguyen, Thi Phuong Trang Phan

Center for Bioscience and Biotechnology, University of Science, Vietnam National University - Ho Chi Minh City,
227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam;
Vietnam National University - Ho Chi Minh City,
Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam

Thi Bich Phuong Chu

Faculty of Pharmacy, Ho Chi Minh City University of Technology,
475A Dien Bien Phu Street, Ward 25, Binh Thanh District, Ho Chi Minh City, Vietnam

Thi Phuong Trang Phan*

Laboratory of Molecular Biotechnology, University of Science, Vietnam National University - Ho Chi Minh City,
Linh Trung Ward, Thu Duc City, Ho Chi Minh City, Vietnam

điểm /   đánh giá
Published
2024-01-25
Issue
Vol. 66 No. 1B (2024): TẬP 6, SỐ 1, THÁNG 1 NĂM 2024
Section
KHOA HỌC KỸ THUẬT VÀ CÔNG NGHỆ