Study on producing a LAMP kit for diagnosis of small liver fluke Clonorchis sinensis infection in humans

Abstract

Background: Clonorchis sinensis is one of the three species of small liver flukes that cause infections in humans. C.sinensis is found mainly in several Asian countries and Northern, Vietnam. The Loop-mediated isothermal amplification (LAMP) with a sensitivity and specificity comparable to that of the PCR method, which requires less complex equipment and facilities for performance. Methods: The research design describes the laboratory experiment and evaluates a LAMP kit for the diagnosis of C. sinensis in the laboratory and the small-scale field assessment, using 150 human stool samples from fascioliasis endemic areas...