Isolation and quantification of ursolic acid from leaves of Syzygium resinosum (Gagnep.) Merr

Abstract

From the ethyl acetate fraction of Syzygium resinosum (Gagnep.) Merr. leaves, using isolation, purification and modern spectroscopic methods such as MS, 1D-NMR, 2D-NMR, a compound was isolated and identified as ursolic acid. The content of ursolic acid was determined by high performance liquid chromatography (HPLC). The results obtained values ​​were 0.46%, 1.66% and 0.35% corresponding to the mass percentage of ursolic acid in the n-hexane, ethyl acetate and methanol fractions of Syzygium resinosum (Gagnep.) Merr. leaves.