Isolation and selection actinobacteria growing on seaweed with the ability to produce alginate lyase
Abstract
Polysaccharide-degrading enzymes have played an important role in discovering of biological functional unsaturated oligosaccharides. Although many such enzymes that can degrade polysaccharides from seaweed have been reported, the enzymes extracted from actinobacteria are rather limited. In this study, there are 20 strains of actinobacteria isolated from brown seaweed, in which strain S1 showed the highest activity of alginate lyase with an alginolytic zone on an alginate agar plate of 55 mm. The optimum culture conditions for strain S1 contained the main ingredients of Sargassum sp. seaweed powder 2% and NaCl 2%; it was incubated at 30oC with sharking at 150 rpm within 6 days. Strain S1 was identified as Streptomyces sampsonii by 16S rRNA sequence analysis, with 99.85% similarity compared with 16S rRNA sequences of actinobacterial species on Genbank.
