ESTABLISHING A RAT MODEL OF CHRONIC KIDNEY DISEASE VIA FIVE-SIXTHS NEPHRECTOMY

Abstract

Objective: To develop a rat model of chronic kidney disease through surgical removal of five-sixths of total renal mass, serving as a foundation for future pharmacological and therapeutic studies.

Subjects and methods: Experimental study on white rats, randomly divided into 10 rats/group, including control group with surgery without kidney removal and given distilled water, model group with surgery to remove 5/6 of the rats' kidneys and given distilled water.

Results: After 2 weeks of surgery to remove 5/6 of the rats's kidneys, in the model group, the concentrations of urea, creatinine, 24-hour urine volume and 24-hour proteinuria of the rats increased compared to before surgery. Histological images of the rats kidneys showed interstitial expansion, glomerular hypertrophy, and tubular dilation. The survival rate of rats was 100%.

Conclusion: The 5/6 nephrectomy model successfully reproduced key clinical and pathological features of CKD with a high survival rate. This stable and reproducible model provides a valuable experimental basis for studying the pathophysiological mechanisms of CKD and for evaluating potential pharmacological or therapeutic interventions in future research.