EVALUATION OF THE ANTIOXIDANT ACTIVITY OF METHANOL EXTRACTS OF SIEGESBECKIA ORIENTALIS L.

Abstract

Siegesbeckia orientalis L. belongs to the family Asteraceae (Asteraceae) is an herbal plant widely distributed around the world and has been used in traditional medicines to treat inflammatory diseases or bone diseases in Asia. In this study, the methanol extract of Siegesbeckia orientalis L. was used for phytochemical analysis and investigation of the antioxidant capacity by three methods including 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS•⁺), 2,2-diphenyl-1-picrylhydrazyl (DPPH), total reducing power assay (PFRAP). The result showed that extract of Siegesbeckia orientalis L. contained alkaloids, flavonoids, phenols, cardiac glycosides, saponins, reducing sugars, and steroids. Moreover, antioxidant potential of Siegesbeckia orientalis L. was concentration dependent with an IC₅₀ value of 77.52 ± 5.4μg/ml (DPPH) and 26.96 ± 1.431μg/ml (ABTS•⁺). All the ABTS•⁺, DPPH radical scavenging activity and PFRAP of Siegesbeckia orientalis L. extract showed the promising antioxidant capacity for further therapeutic use.