STUDY ON THE IN VITRO PROPAGATION OF Anoectochilus sp.

Abstract

Anoectochilus sp. is a medicinal plant with many valuable healing properties. It has long been used in traditional medicine as a remedy for ailments such as stomach pain and high fever. However, due to overexploitation, this species is now endangered in the wild. This article investigates the influence of auxin compounds and substrates on the growth of Anoectochilus sp. to develop an in vitro rapid propagation. The results showed that the Gamborg B5 medium supplemented with 4 mg/L indole-3-butyric acid (IBA) yielded the best results, with the average number of shoots, leaves, and roots per original sample reaching 2.56 shoots/sample, 5.00 leaves/shoot, and 4.33 roots/shoot, respectively. The mixed substrate in the treatment, consisting of pine bark (40%), coconut fiber (10%), burnt rice husk (10%), soil (10%), and cow manure (20%), was suitable for the growth of Anoectochilus sp. seedlings. Seedlings grown on this substrate reached a height of 7.72 cm with 5.00 leaves/plant and 3.20 roots/plant, respectively, and a survival rate of 100% after 12 weeks of planting. This research has provided a valuable scientific and practical basis, laying the foundation for breeding, conservation, and the future development of this orchid species