STUDY ON EXTRACTING MUGWORT ESSENTIAL OIL, DETERMING CHEMICAL COMPOSITION AND INVESTIGATING ANTI-BACTERIAL ACTIVITY

Abstract

Mugwort essential oil has a lot of uses in medicine and especially capable of treating cancer. This study aimed to optimize the technical parameters for the extraction of essential oil from Mugwort leaves and determine the physicochemical index and antibacterial activity. Using the distillation method that attracts steam directly in the Clevenger light essential oil distillation unit. Experiment arrangement by random method, repeated three times. Data processing using SAS 9.4 software. The results showed that the old leaf material, cut 1cm, soaked for 30 minutes in 10% NaCl, distilled 180 minutes, wilted for 120 hours, the ratio of raw material: distilled water was 1:3, suitable for collection. Essential oils from Mugwort leaves. The essential oil content is 0.482%. Physical and chemical index: density is 0.885; IA: 3,630; IE index: 36,881; IS index: 40,486; Ethanol index: absolute oil/alcohol ratio = 1:10.47; the ratio of essential oils/alcohol 90^o = 1:14.27; the ratio of essential oils/alcohol 80^o = 1:23.90; The ratio of essential oil/alcohol 70^o = 1:48.43. Mugwort oil is resistant to bacterial strains (Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Shigella boydii). The parameters of Mugwort oil collection have been optimized. Determination of wormwood essential oil content grown in Binh Duong and determination of the physicochemical index of essential oil. Mugwort leaf oil is resistant to gram (+) and gram (-) bacteria strains.