BREEDING OF DOUBLE FLOWER HIPPEASTRUM (Hippeastrum sp.) BY BULB CUTTING

Abstract

The double hippeastrum is being interested in by flower players, but the bulb supply is limited and the bulb’s price is expensive. Therefore, researching clonal propagation with a fast multiplier and short time to meet the demand for the double hippeastrum for our country is an urgent requirement. Soaking the split tubers in BAP with different concentrations for 30 minutes, results showed that BAP 2.0 mg/l was suitable for bud regeneration the slicing pieces method; the scale of bulb method was 1.0 mg/l and the slicing 2/3 bulb method was BAP 2.5 mg/l. Soaking the split tubers in BAP combined with α-NAA at different concentrations for 30 minutes, the results showed that BAP 2.0 mg/l combined with α-NAA 0.6 mg/l was suitable to creat multiple shoots of the slicing pieces method, reaching 4.31 shoots/sample; the scale of bulb method BAP 1.0 mg/l and α-NAA 0.6 mg/l was the best reaching 3.91 shoots/sample; the slicing 2/3 bulb method BAP 2.5 mg/l with α-NAA 0.6mg/l was the best reaching 4.21 shoots/sample. The shoots of the split tubers were sprayed with different concentrations of α-NAA at a frequency of every 5 days, the results showed that α-NAA 0.4 mg/l was the most suitable for rooting of slicing pieces and slicing 2/3 bulb methods; the scale of bulb method α-NAA 0.6mg/l was the most suitable. Tribat medium was the best for the growth of the double hippeastrum.