OPTIMIZE THE EXTRACTION PROCESS OF FLAVONOID FROM BOHENIVEA L. GAUD IN CAO BANG PROVINCE

Abstract

The purpose of this study was to investigate the one way ethanol concentrations, time, temperature and the ratio of material/solvent extraction, which affected the extraction process of flavonoids from Boehmeria nivea (L.) Gaud, resulting in 70%; 90 minutes; 80°C; 1/15 (w/v). On the basis of the factors affecting the extraction conditions, we found that the solvent concentration, extraction time and material/solvent ratio strongly influence the extraction process. Application of Box-Behnken design found the optimal condition for the extraction process of Boehmeria nivea (L.) Gaud with ethanol at concentration of  71.33%, extraction time 94 minute, material/solvent ratio 1/15.67 which created the amount of flavonoids of 36.17%. Experimental results showed high degree of compatibility with the model.