PROTOCOL FOR MULTI – SHOOT REGENERATION OF DIEP HA CHAU (Phylianthus urinaria L.)

Abstract

Hepatitis B is one of the most deadly infectious diseases in the world. Diep Ha Chau is a medical plant used for treating many human’s diseases, especially Hepatitis B. Diep Ha Chau contains phyllanthin, hypophyllathin and triacontanal which are able to detoxify and recover normal function of the liver, even in cases of liver impairment due to alcohol abuse. These substances increase the amount of glutathione - a liver protecting agent that is severely lack in patients who regularly use alcohol. In this paper, we indicate “the multi-shoot regeneration capacity” of Diep Ha Chau to increase gene transferring efficiency. First, sterilize seeds in “javen” 60% in about 10 minutes, the suitable environment for shoot propagation and growth is basic MS added BAP 1 mg/l; pH = 5.8. The Suitable environment for complete plant formation is basic MS added NAA 0.5 mg/l; pH = 5.8.