MAIZE TRANSFORMATIONAND DETERMINATION OF ZmbZIP72TRANSCRIPTION FACTOR GENE IN T0 PLANTS

Abstract

Studies have shown that the ZmbZIP72 gene is of significant importance to the drought tolerance of plants as well as to maize. In this study, the expression vector of plants carrying ZmbZIP72 gene under the control of RD29A stress-induced promoter was transferred into maize. Transgenic experiments were conducted in autumn-winter crops 2016 and 2017. The transgenic maize plants were fully developed in the tissue culture laboratory then grown up in the greenhouse for self - pollination. Then, PCR to amplify ZmbZIP72 and Hyg genes was used to test the presence of transgenes in maize plants. The results shown that, in autumn-winter of 2016, 44 transgenic plants survived in the greenhouse therein 2/44 were positive with Hyggene. In the autumn-winter crop of 2017, there were 140 transgenic plants surviving with 17 positive plants which were verified with ZmbZIP72 primers and Hyg primers. In conclusion, the authors have obtained T0 transgenic plants, the next generations will be genotype evaluated in following season crop.