STUDY ON THE PREPARATION OF NANOPARTICLES CHITOSAN-PEG ENCAPSULATE Cordyceps militaris Link EXTRACT

Abstract

Cordyceps sp. has been uded in tradtional medicine for a long time in many Asia countries. Currently, many species of this family have been successfully cultivated on artifical medium to create a stable resources and help to preserve natural Cordyceps. Nanotechnology has been widely used in medicine to increase the pharmacological effectiveness of drugs. This study was successful in extracting the bioactive compounds from  C. militaris Link NBRC 100741 fruiting bodies according to the fractional extraction procedure (ethanol 96^o, ethanol 50^o, water) at 55^oC, resulted in a extract with cordycepin content at about 9.92 mg/g extract, adenosine content reach 1.32 mg/g extract, moisture <11%. Nanoparticles chitosan-PEG-Cordyceps extract were successfully prepared when combined chitosan and PEG at ratio 70/30, reaction temperature at 70^oC, stirring speed at 900 rpm. Packaging efficiency reached 14.61% for cordycepin and 80.82% adenosine after 24 hours of reaction.