α-GLUCOSIDASE INHIBITORY ACTIVITY OF THREE GREEN SEAWEEDS COLLECTED IN THE COAST OF KHANH HOA PROVINCE

Abstract

One of the effective therapeutic approaches in the management of type 2 diabetes is inhibition of α-glucosidase. This study investigated α-glucosidase inhibitory activity of three green seaweeds (Halimeda macroloba, Ulva reticulate and Ulva lactuca) and the results indicated that the inhibitory effect (IC₅₀ values) of H. macroloba, U. reticulata and U. lactuca and were 3.98, 4.76 and 5.21 mg/ml, respectively. The suitable extraction time and extraction temperature for extracting high activity α-glucosidase inhibitors from green seaweed H. macroloba were found to be 60 min and 60 °C, respectively. Among the extract fractions, ethyl acetate showed the highest α-glucosidase inhibitory activity (IC₅₀ value of 2.45 mg/ml), followed by n-hexane, butanol and water with IC₅₀ values of 2.79, 4.11 and 4.91 mg/ml, respectively. Phytochemical screening showed phenolic, flavonoids and terpenoid were present in the ethyl acetate fraction of green seaweed H. macroloba.