SCREENING AND IDENTIFICATION OF ACTINOMYCETE SHOWING ANTIFUNGAL DISEASES ACTYVITY IN PLANT

Abstract

Our aim to find potential Streptomyces species producing antifungal compound for biocontrolling fungal diseases of plants. Strain P5-1 have the strongest ability to inhibit both Fusarium oxysporum and Phytophthora capsici among the 379 actinomycetes isolated previously. Total antifungal compound is yellow color, well soluble in water and inhibits growth of Fusarium oxysporum and Phytophthora capsici ranging from 8 and 16 µg/ml, respectively. Spores of strain P5-1 are straight, ellip-shape, smooth surface, spore size of 0.8 × 1.0 µm. Analysis of 16S rRNA gene sequence of strain P5-1 confirmed that strain P5-1 is a member of the genus Streptomyces, has the highest similarity (99.64%) to Streptomyces pratensis ch24^T (JQ806215), was named as Streptomyces pratensis P5-1 (MK652886). Strain P5-1 could considered a potential candidate for development of probiotics application for treating fungal pathogens in plants.