CHARACTERIZATION OF ACTINOMYCETES STRAIN TA3.A03 ISOLATED FROM MARINE SEDIMENT OF VIETNAM

Abstract

Marine-derived Streptomyces are a source of a broad variety of secondary metabolites with diverse biological activities. A TA3.A03 strain isolated from marine sediment was studied characterization biology classified using a 16S rRNA. The strain TA3.A03 is capable of extracellular enzymes producing, such as cellulase, protease, amylase, and lipase. The isolate exhibited a broad spectrum of antimicrobial activity against the pathogenic bacteria used in the study. The isolate was in the grey aerial mycelium colour series on all tested media with long spore chains which are non-motile spores. Aerial mycelium and substrate mycelium were well developed but not fragmented in most media. Growth of the isolate occurred in 1-7% (w/v) NaCl at pH 5.0-9.0 and 20-45°C. The organism, isolate TA3.A03, was found to have chemical and morphological properties typical of members of the genus Streptomyces. The isolate was shown to be closely related to the type strain of Streptomyces zhihengii strain INA 766 (98.19 % similarity). Based on the physiological, biochemical and chemotaxonomic tests, the actinomycetes was identified as an isolate of Streptomyces sp. TA3.A03.