STUDY ON THE VIRULENCE OF ISOLATED SALMONELLA STRAINS FROM CHICKENS INFECTED WITH SALMONELLOSIS AND USE OF NANOSAN PROBIOTICS IN TREATING THE DISEASE FOR CHICKEN AT TAM DUONG DISTRICT, VINH PHUC PROVINCE

Abstract

This study investigates the prevalence of diarrhea in chickens raised in households and farms in 3 communes: Thanh Van, Duy Phien, Kim Long of Tam Duong district, Vinh Phuc province. The results showed that, 3.69% of chickens had diarrhea at 1-6 weeks old, 4.10% at 7-20 weeks old and 6.55% of chickens over 20 weeks old; 6.60% of chickens had diarrhea in the spring-summer season and 2.89% of chickens had diarrhea in the autumn-winter season; 6.70% of chickens raised in open cages and 2.92% of chickens in closed cages have diarrhea. Testing the virulence of 20 strains of Salmonella gallinarum pullorum isolated by PCR method, there were 20/20 strains (100%) carrying Stn and InvA genes; none of the strains carried the DT104 factor. Using randomly 3 strains of Salmonella gallinarum pullorum to be injected into 6 white mice, all 6/6 (100%) mice died from 8 to 20 hours after injection. The results of the antibiogram test showed that norfloxacin and enrofloxacin were most sensitive to Salmonella bacteria (the average antibiotic disk varied from 26 to 34 mm). Using enrofloxacin in combination with electrolytes (herbal K-C glucose) to treat salmonellosis, there was 90.00% of chickens recovered from the disease; using enrofloxacin in combination with electrolytes (herbal K-C glucose) and NanoSan probiotics to treat salmonellosis, there was 93.33% of chickens recovered from the disease.