EXTRACTION AND ISOLATION OF BEAUVERICIN FROM CORDYCEPS CATENIANNULATA CPA14V

Abstract

Beauvericin is a well-known mycotoxin produced by Beauveria and many other entomopathogenic fungi, including Coryceps species. The strain Cordyceps cateniannulata CPA14V was isolated in Vietnam and it was first record of beauvericin produced by the species. Beauvericin exhibits various bio-activities including supporting cancer treatment, preventing infection from viral and bacterial. The study established a procedure for extracting and isolating Beauvericin (purity 98.1 %) from Cordyceps cateniannulata CPA14V by using combination of different chromatographic methods such as: thin layer chromatography, normal-phase chromatogaraphy with silicagel stationary phase, reversed-phase chromatography with YMC RP 18 stationary phase and size exclusion chromatography with sephadex LH-20 stationary phase at laboratory scale. Extraction conditions were at 40 - 50^oC, sonication for 2 hours, used dichloromethane solvent. The results showed that Cordyceps cateniannulata CPA14V has great potential for culture, extraction and isolation Beauvericin for further research and development into medicinal products, contributing to community health care.