STUDY ON CREATING Agrobacterium tumefaciens STRAINS HARBORING PKO2-PR1 VECTOR FOR PR1 GENE DELETION IN Verticillium dahliae

Abstract

Verticillium dahliae is a noxious fungal pathogen that affects plants. It can cause wilting and premature senescence in many crops, seriously affecting crop productivity. The PR1 gene has been mentioned in many previous studies, which may be related to the initial stage of the host infection process. To serve research on the role of the PR1 gene in V. dahliae, a strain of A. tumefaciens bacteria containing the pKO2-PR1 vector was created. The vector pKO2-PR1 design model was successfully created using Snapgen Viewer software. The pKO2-PR1 vector contains two segments of sequence before and after the PR1 gene (1.8 kb). These segments were amplified by PCR and cloned into a recombination vector. The resulting recombined vector was verified by using the XhoI restriction enzyme. The sequence of the pKO2-PR1 vector was fully consistent with the initial design model. The pKO2-PR1 vector was successfully transferred into A. tumefaciens bacteria. The A. tumefaciens strain carrying the pKO2-PR1 vector is an important tool for gene deletion and functional study of the PR1 gene in V. dahliae.