IN VITRO ANTI-OXIDANT, ANTI-HEPG2 PROPERTIES OF AQUEOUS EXTRACTS FROM VARIOUS PARTS OF Ludwidgia hyssopifolia
Abstract
Ludwigia hyssopifolia is traditionally used in the treatment of jaundice in Asian countries. There were no reports on the plant for in vitro hepatoprotective property in Vietnam. This study was aimed to evaluate the aqueous extracts of four various parts (whole plant, leaves, stems and roots) of L. hyssopifolia for its in vitro anti-oxidant and hepatoprotective activities. Medicinal plants were authenticated by DNA barcoding technique. Preliminary analysis of phytoconstituents, total polyphenol and flavonoid contents were also conducted. The antioxidant potential of the 04 extracts was investigated by employing in vitro DPPH assay while the hepatoprotective activity was assessed by calculating in vitro growth inhibition percentage on HepG2 cells. The results showed aqueous extract of whole plant contained flavonoids, saponins, tanninoid, reducing sugars and polyuronid. Leaf and whole plant extracts displayed the highest total polyphenol contents (148.30 ± 0.80 and 136.23 ± 1.00 mg GAE/g) as well as total flavonoid contents (30.34 ± 5.74 and 23.56 ± 8.81 mg QE/g). Leaf and whole plant aqueous extracts also exhibited the greatest antioxidant activity (IC50 of 45.27 ± 0.92 µg/ml and 46.00 ± 0.78 µg/ml). Whole plant aqueous extract showed the highest anti-HepG2 activity (IC50 of 176.3 ± 5.12 µg/mL). Aqueous extract from Ludwigia hyssopifolia whole plants is potential sources in the development of hepatoprotective medications.