VALIDATION OF BIOCHIP-BASED E.COLI DETECTION VIA RAMAN SPECTROSCOPY

Abstract

  The study focuses on validating the capability to detect E.coli DNA based on the Raman spectroscopy method. The sensor, fabricated on a nano-material-coated substrate, was characterized using SEM and EDS techniques, and the E.coli DNA detection process was evaluated through Raman scattering analysis. This study successfully synthesized AgNPs/ZIF-8 with an average size of ~ 700 nm, confirmed by SEM and EDS analysis, showing the presence of C (37.22%), N (23.54%), Zn (21.04%), and Ag (9.64%). The material was coated onto glass substrates, exhibiting a size range of 700 - 1.000 nm and elemental composition of C (37.63%), N (28.08%), Zn (29.43%), and Ag (2.33%). Raman spectroscopy revealed probe signals (480 and 780 cm⁻¹) and E.coli DNA signals (560 and 1.094 cm⁻¹), with hybridization amplifying intensity by 2.4 - fold. Optimal DNA detection was achieved at 50 ng/mL (signal intensity: 274), demonstrating the system’s efficacy for biosensing applications.