Quantification of different drugs in the treatment regimen for Helicobacter pylori infection using high performance liquid chromatography (HPLC) method

Abstract

    The research has applied the HPLC-PDA method to simultaneously quantify six types of drugs used in the treatment of Helicobacter pylori infection, including famotidine, metronidazole, levofloxacin, sodium rabeprazole, sodium pantoprazole, and lansoprazole. The separation was performed using a C18 reverse-phase column (250 mm × 4.6 mm, 5 μm) and a mobile phase consisting of a phosphate buffer solution at pH = 5.0 and acetonitrile, flow rate of 1 mL/min using a gradient elution program. The gradient elution started with a phosphate buffer:acetonitrile ratio of 9:1 (v/v), which was then changed to reach a ratio of 4:6 (v/v) over 25 minutes. The quantification of the analytes was based on the peak area measurements of famotidine at 267 nm, metronidazole at 320 nm, levofloxacin at 294 nm, and sodium rabeprazole, sodium pantoprazole, and lansoprazole at 284 nm. The validation of the analytical procedure, including system suitability, linearity, specificity, repeatability, and accuracy, met the requirements of the International Conference on Harmonization (ICH) guidelines. As a result, this analytical approach can be used to routinely monitor the quality of these chemicals in pharmaceutical dosage forms.