Detection and genetic characterization of Tomato Spotted Wilt Virus (TSWV) infecting chrysanthemum in Lam Dong province

Abstract

Tomato Spotted Wilt Virus (TSWV) is an important infectious disease that infects many crops and causes serious economic lose for the argriculture all aroud the world. In this study, TSWV- suspected chrysanthemum samples were collected from Lam Dong province, Vietnam. RT-PCR was used, successfully amplifying a specific 777 bp N gene segment of the TSWV. Genetic characterization of the N gene revealed that the studied TSWV strains shared highest nucleotide sequence idetity (96.5%-99.6%) with the strains isolated from India, Venezuela, Zimbabwe, New Zealand, Japan and Korea, suggesting that these strains shared common ancestor and for that the Vietnamese TSWV may infected from TSWV strains isolated in one of those countries. These results provided important information on the biological and molecular characterization of the circulating TSWV in Vietnam, as well as the evident for control and predict the circulation of TSWV in chrysanthemum in future.