Procedure for Isolation of Chemical Composition from Do Bau Leaves (Aquilaria crassna Pierre leaves)

Abstract

The paper presents the chemical compound isolation procedure from Do Bau leaves that were picked in Binh Duong province.  2,5kg of dried leaves was collected from 7kg fresh leaves. Next, the dried leaves were extracted three times with methanol via reflux method in two hour and then all extracted solution went through low pressure evaporation to get methanol gel. This methanol gel was then extracted using ethyl acetate to get the ethyl acetate gel that was then studied using column chromatography (on silica gel) with different polarization solutions. Finally, C1-C5 segments were obtained. C2 segment was selected for study  by column chromatography to get the compound CC2. The compound structure was determinized through analysis of their NMR-spectroscopic data with ¹H, ¹³C NMR, 2D-NMR HSQC and HMBC. The compound was named 7-O-metilapigenin 5-O-[β –D-xylopyranosyl-(1→6)- β-D-glucopyranoside]