Extraction and stability of anthocyanin in butterfly pea flower (Clitoria Ternatea L.).

Abstract

    This study was aimed to investigate the extraction and evaluate the durability of anthocyanin from Clitoria ternatea L.flower. To maximize the extraction yield, the appropriate extraction conditions were as follows: solvent: EtOH 50 %; material/solvent ratio: 1/9; extraction temperature: 50 ⁰C; extraction time: 30 minutes; number of extraction steps: 2 and CT flower collection time at 7 a.m. Under these conditions, the amount of anthocyanin was 76.41 mg/L corresponding with   2.19 mg anthocyanin/g dried material. The anthocyanin durability of extract and residue was evaluated in two conditions: at room temperature and at 45 ⁰C. It turns out that the temperature had a significant effect on anthocyanins and polymeric anthocyanin, samples with citric acid (1 – 3) g/L were more stable than those without citric acid. In DPPH scavenging assay, the IC₅₀ of C.ternatea flower residue was 400 µg/mL (with y= 0.1565x – 12.965, R²= 0.9939) compared to the IC₅₀ of ascorbic acid (7 µg/mL), and that figure of CT flowers was lower than the IC₅₀ of vitamin C by approximately 57 times. These results showed that CT flower has antioxidant potential which provide a basis for the use of the plant and can be harnessed as drug formulation.