The HPLC method developement for determination of hydroxychavicol in betel (Piper Betle L. piperaceae) extract

Abstract

   The betel extract contains hydroxychavicol which was evaluated for antidermatophyte and antibacterial activity. An HPLC procedure was developed for determination of hydroxychavicol in the extracts of betel: column C18 5 µm (2,1 mm x 250 mm), mobile phase – acetonitril : orthophosphoric acid 0.05 % (30 : 70), flow rate of 0.5 mL/min, injection volumn of 2 µL. By validation, the proposed method proved quite adequate compatibility, satisfactory specificity and desirable linearity (R² = 0,999). The RSD value was 1.12 %, the recovery percentage was 96 %, the detection limit was 0.130 ppm and the quantitation limit was 0.394 ppm. The validated method was used for quantitative analysis hydroxychavicol content in the dichloromethane betel extract (85 %).