Investigation of fungicidal activities of phyllanthus amarus, allium sativum, and azadirachta indica against leaf atrophy in Aloe vera

Abstract

     Leaf atrophy caused by Curvularia sp. in Aloe vera is one of the significant negative impacts on crop yields and quality. Therefore, screening and determining the fungicidal and fungistatic activities of natural extracts are potential to inhibit and exterminate the pathogenic fungi as the causal agent. This study was conducted to determine the fungicidal activities against Curvularia sp. of three plants, including Phyllanthus amarus, Allium sativum, and Azadirachta indica. The extracts with different polar solvents were yielded by liquid-liquid extraction method. Disk-diffusion and broth microdilution methods were used to determine the fungicidal activities of plant extracts.
    The treatment of the hexane extracts of Allium sativum and Azadirachta indica can produce an antibacterial ring against the activity of the fungus Curvularia sp. The minimum inhibitory and minimum antifungal concentrations MIC and MFC) of A. sativum and A. indica hexane extracts were both 2.5 mg/mL and 5 mg/mL, respectively.
     These results provided a potential capability against Cuvurlaria sp. of A. sativum and A. indica hexan extracts, bringing a promising opportunity for further study due to the development of anti-fungal products applying in Aloe vera crop.