Investigation of extraction conditions for obtaining an potential antioxidant extract from the leaves of Symplocos cochinchinensis (Lour.) S. moore
Abstract
"Dung" (Symplocos cochinchinensis) is a woody plant species commonly found in the Central and Tay Nguyen regions of Vietnam. The leaves of S. cochinchinensis are often used in traditional medicine to support the digestive system and treat indigestion and menstrual disorders. Several studies have demonstrated that leaf extract has many valuable activities, including lipid-lowering, antioxidant, cytotoxic, and hypoglycemic properties. The study collected and identified Dung leaf samples from Tra Bong district, Quang Ngai province, by using the DNA method. This research investigated the extraction conditions for obtaining the most effective antioxidant extract from S. cochinchinensis leaves. Serveral solvents were used for screening method, including water, ethanol (EtOH) 50 %, 70 % and 96 %. At the same time, supporting conditions such as ultrasound and temperature are also considered. The results showed that extraction with 70 % EtOH and ultrasound for 20 minutes at 40 oC produced the extract with the best antioxidant activity (IC50 = 439.99 ± 14.58 µg/mL). This extraction process can serve as a premise for developing products from Dung leaves.
