Simultaneous quantification of the compounds ascorbic acid, acetaminophen, phenylephrine HCl, guaifenesin, and dextromethorphan HBr in tablets using high-performance liquid chromatography

Abstract

    The present study was conducted with two objectives: (1) construction and validation of a simultaneous quantitative process for ascorbic acid, acetaminophen, phenylephrine HCl, guaifenesin, and dextromethorphan HBr in tablet form using the HPLC method, and (2) application to quantify some commercial products containing these compounds. The chromatographic separation of these five pharmaceutical compounds was performed on a C18 reverse-phase column (250 mm × 4.6 mm, 5 μm), using a gradient elution program with mobile phases A (phosphate buffer pH = 3.0) and B (acetonitrile). The research results obtained linear concentration ranges for ascorbic acid, phenylephrine HCl, acetaminophen, guaifenesin, and dextromethorphan HBr as (8-56, 16-104, 40-240, 16-80, and 24-146) µg∙mL⁻¹, respectively. The accuracy of the method was determined by recovery rates in the range of (98.80-101.27) %. The repeatability with the relative standard deviation (% RSD) was 0.64 %, which is less than 2.0 %. The proposed method can effectively quantify six types of commercial tablets, all of which meet the content requirements of the Vietnamese Pharmacopoeia V.