Study on phytochemistry screening and biological activities of Ethanol extract of Stenochlaena palustris (Burm. f.) Bedd

Abstract

     For a long time, Stenochlaena palustris (Burm. f.) Bedd has been commonly used as an edible vegetable, with limited studies on chemical composition and biological activities. In the present study, the 96 % ethanol extract of Stenochlaena palustris was obtained using ultrasound extraction method to analyze its chemical composition and biological activities. The antioxidant effect was tested by using the DPPH assay and the inhibitory effect on α-glucosidase was evaluated by using acarbose as a positive control. The anti-inflammatory activity was tested by inhibiting nitric oxide (NO) production ability. The results showed that the 96 % ethanol extract showed strong α-glucosidase inhibitory activity with IC₅₀ = 19.54 µg/mL. The antioxidant activity was assessed through DPPH radical scavenging assay with IC₅₀ = 801.60 µg/mL. The extract demonstrated low anti-inflammatory activity, as indicated by a 7.39 % inhibition of nitric oxide (NO) production in macrophages at a treatment concentration of 1.56 µg/mL.The study showed that S. palustris exhibited weak antioxidant and anti-inflammatory properties but demonstrated significant potential in α-glucosidase enzyme inhibition. These results provide a scientific basis for further investigation into the potential application of this herbal in diabetes treatment.