Reverse phase liquid chromatography: quantitative application of ticagrelor tablets (Rp-HPLC)

Abstract

    In this study, ticargrelor tablets were quantified via reverse phase liquid chromatography (Rp-HPLC). Chromatographic conditions were investigated and optimal parameters were selected as following: wavelength, mobile phase, stationary phase, flow rate, and sample injection volume. The developed analytical process is evaluated in accordance with the guidance of Circular 32/2018/TT-BYT regulating the registration of circulation of drugs and medicinal ingredients. Results of chromatographic conditions for quantification of ticagrelor tablets are: Pursuit XRs C18 column (150 x 4.6 mm; 5 µm); Detection wavelength at 301 nm; The mobile phase being a mixture of acetonitrile and 0.1 % phosphoric acid with the ratio of 77:23 (v/v); Flow rate at 1 mL/min; Sample injection volume 10µL and column temperature 40 ⁰C. Ticagrelor linear range is set from (5 – 50) µg/ml (Correlation coefficient R² > 0.999). The Rp-HPLC method for ticagrelor determination has been successfully developed and validated (linearly, correctly, and accurately) and can be applied in the content determination of ticagrelor tablets as a baseline standard.