The study on anti – Candida activities of Piper betle’s extract in dependence and combination with miconazole

Abstract

    In recent years, Candida spp. has a high rate of resistance against miconazole. To achive a therapeutic effect, it is necessary to increase either the dose or the frequency of administration of miconazole, but it can cause many side effects on the liver and kidneys. Therefore, studies have tended to focus on natural medicinal products to recplace or combine with chemical medicines to solve this problem. This study was conducted to assess the antifungal activity of the betle - extract in independence and combination with miconazole on Candida spp. The process of extraction and purification from betle leaves were studied using cheap, and safe sovents (alcohol, water). Via agar microdilution method in MHA added with 2 % glucose, the purified extract had an MIC value of 2000 µg/m: on the 4 strains of C. albicans ATCC 10231, C. albicans 01, C. krusei 8TX and C. tropicalis 58. With checkerboard assay, the combination of purified betel extract and miconazole demonstrated synergistic effect and additive effect on 2 strains of C. albicans, and separated effect on the other 2 strains.