STUDY ON THE CATALYTIC AND H2O2 SENSING CAPABILITY OF GOLD NANOPARTICLES SYNTHESIZED FROM THE EXTRACT OF PHLOGACANTHUS TURGIDUS

Abstract

In this study, the AuNPs synthesized from the extract of Phlogacanthus turgidus exhibited absorption peaks
within the wavelength range of 500 nm to 600 nm. The results of TEM and SEM showed that the structure of
AuNPs exhibits various shapes, including cylindrical, spherical, and polygonal forms. Additionally, clusters
of AuNPs were observed with different sizes. Various factors affecting the redox reaction between hydrogen
peroxide (H2O2) and 3,3',5,5'-Tetramethylbenzidine (TMB) using AuNPs as a catalyst were investigated.
These factors included temperature, reaction time, pH, the amount of AuNPs, TMB concentration, and H2O2
concentration. UV-Vis spectrophotometry was utilized to determine the optimal conditions for H2O2
detection through the ox-TMB color reaction with the AuNPs catalyst, thereby evaluating their applicability
as H2O2 sensors.