ANALYSIS OF CHLOROGENIC ACIDS IN THE GREEN COFFEE BEANS OF DIFFERENT COLLECTION TIMES BY HPLC-QTOF TECHNIQUES

Abstract

Chlorogenic acids (CGAs) constitute a large family of esters and are present in many medicinal plants.
CGAs play an important role in modern traditional medicine, but only some of this family’s pure compounds
have been thoroughly researched and shown good pharmaceutical effects. The green coffee beans (CFX)
contained ~ 11% of CGAs depending on the species, cultivation and harvested conditions. The quatitative
analysis could show the presence of separate CGA compounds. Instead of using expensive pure CGA
compounds as analysis standard, the HRMS-QTOF detector could unequivocally assign the compound
identity while the coupled HPLC could effectively separate the CGAs . In this study, the HPLC-QTOF
technique was used to qualitatively determine the CGAs present in the green coffee beans. Ít showed that the
CFX harvested in different times have the same CGAs profiling.