PREPARATION AND EVALUATION OF IN VITRO ANTIFUNGAL BIOASSAY OF BOTANICAL NANOEMULSIONS AGAINST SCLEROTIUM ROLFSII AND RHIZOCTONIA SOLANI PHYTOPATHOGENIC FUNGI

Abstract

In this study, the nanoemulsion containing ethyl acetate extract of Polygonum cuspidatum root was
successfully prepared. The emulsified extract was dispersed in an aqueous medium at a dilution ratio of
400-800 times to form nanoemulsions. The resulting nanoemulsions had an average particle size of 219 to
227.9 nm based on dynamic light scattering (DLS) measurement. Fourier transform infrared
spectroscopy (FTIR) was also used to evaluate the composition and quality of the extract in the product.
In vitro antifungal bioassay of the obtained nanoemulsions on fungal strains Sclerotium rolfsii and
Rhizoctonia solani, showed excellent inhibitory effects. The inhibitory effects ranged from 84.88% to
100% for S. rolfsii and R. solani in vitro. Therefore, the nanoemulsions containing the plant extract
promised to bring outstanding effects in developing plant protection products in the future.