RESEARCH ON DETERMINATION OF AROMATIC AMINES RELEASED FROM AZO DYES IN LEATHER, IMITATION LEATHER BY USING HPLC-MS/MS METHOD

Abstract

    An analytical procedure based on the use of high-performance liquid chromatography tandemmass spectrometry/mass spectrometry (HPLC-MS/MS) was applied for the determination ofbanned azo dyes in leather and imitation leather. For the identification of the analytes oneparent ion and two product ions were selected and the LC-MS/MS parameters optimized toobtain high sensitivity and selectivity. This method be able to determine 18 aromatic aminesderived from azo colorants using C18 column and using MeOH: H2O as a mobile phase.Sample was extracted in 17 mL of 0.06 M citrate buffer solution at pH 6 at a temperature of70 °C. Then, the extracted colorants were reduced to aromatic amines using 3 mL of 200mg/sodium dithionite solution. After an incubation of 30 min at 70°C, the mixture was allowedto cool to room temperature. The amines released in the process of reductive cleavage waretransferred to a t-butyl methyl ether phase by means of liquid-liquid extraction, the t-butylmethyl ether extract was concentrated and was then filtered with syringe filters of 0.45-μmpore size. Method detection limit was 0.5 mg/Kg.