APPLICATION OF MULTIPLEX–PCR TECHNIQUE FOR DETECTION OF VIBRIO CHOLERAE, VIBRIO PARAHAEMOLYTICUS AND THEIR VIRULENCE GENES FROM SEAFOOD

Abstract

    The objective of this study were to compare the ability to detect V. cholerae and V.parahaemolyticus by conventional methods using TCBS (Thiosulfate Citrate Bile SaltsSucrose), CAV (CHROMagar^TM Vibrio) medium and PCR method; and also to identify thepresence of virulence genes of V. cholerae, V. parahaemolyticus from fresh seafood by usingm-PCR technique. A total of 313 samples (126 from fillet fish, 52 from shrimp and 135 fromclam) were collected at Center of analytical services and experimentation in HCMC.

PCRKeywords: Vibrio cholerae, Vibrio parahaemolyticus, virulence gene; fresh shrimp, fish filletand clam product.