DEVELOPMENT OF ALKALINE PHOSPHATASE BIOSENSORS BASED ON ELECTRODES MODIFIED WITH MULTIWALLED CARBON NANOTUBES FOR THE DECTERMINATION OF PATULIN

Abstract

   Amperometric biosensors based on platinum screen-printed electrodes modified withmultiwalled carbon nanotubes (MWCNTs) and immobilized enzymes alkaline phosphatasedeveloped for the determination of patulin. Biosensor used in patulin detection based on theinhibition of enzymatic activities. The working concentration range is 110-6 -110-10 mol/l,limit of quantitation is 510-11 mol/l. Modification of electrode with MWCNT allows achievingwider range of detectable concentrations, lower detection limits, and more pronounced effectof inhibition.

Keywords: patulin, alkaline phosphatase, mycotoxins, carbon nanotube, amperometricbiosensor.