HYDROLYSIS GAMMA ORYZANOL BY CO-SOLVENT METHOD AND SIMULTANEOUS DETERMINATION OF PRODUCTS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

      In this study, we investigated the preparation of ferulic acid, one of potential antioxidants which is widely used in the pharmaceutical industry, and cosmetics, from the hydrolysis of gamma oryzanol (γ-oryzanol), compounds containing by-product of rice bran oil processing. In alkaline solution using co-solvent, γ-oryzanol hydrolysis reaction has been studied and examined in terms of the different rates of alkaline catalysts, temperature, and ultrasonic (20 kHz). We investigated suitable analysis conditions to simultaneously analyze the product of the γ-oryzanol hydrolysis reaction, optimized hydrolysis conditions to obtain high efficiency. In heat treatment condition, with the intial concentration of γ-oryzanol of 6 mg/mL, and KOH/ γ-oryzanol ratio is 20:1 (wt/wt), the reaction at 75 oC got the efficiency of 83.3%. Meanwhile, at 60 oC using the ultrasonic assistance (20 kHz, 180 W), the efficiency was reached up to 93.3% in just 2.5 hours. The aim of this work is to make a basic data for the ferulic acid production research process from soapstock, a by-product that has high γ-oryzanol concentration.

Keywords: ferulic acid, γ-oryzanol, ultrasonic, hydrolysis reaction, co-solvent