APPLICATION OF ELECTROCHEMICAL BIO-SENSOR BASED ON MODIFIED MICRO-ELECTRODE TO DETECT PLANT PROTECTION DRUGS

Abstract

In this study, electrochemical biosensors based on conductive polymer (polyaniline, PANi) and carbon
nanomaterials (graphene, Gr) were fabricated on a screen-printed electrode (Pt-SPE) to detect plant
protective drugs (Methamidophos). PANi films were synthesized onto microelectrodes using cyclic
voltammetry (CV) then coated Gr film directly onto the electrode. The PANi/Gr bilayer hybrid material
has good electrical conductivity, high porosity, good adhesion to biological molecules, and fast
electronic transmission. PANi/Gr hybrid membranes were used to fabricate disposable
acetylcholinesterase sensors to detect acetylthiocholine (ATCh). The electrochemical sensor is capable
of detecting the Methamidophos with a detection limit of 1-50 ppm for a period of 10 minutes with the
error permitted limit (15%). The electrochemical sensor provides a very promising technical solution to
monitor topical acetylthiocholine levels in patients with neurological diseases and to identify
neurotoxins such as sarin and pesticides.