SYNTHESIS OF ANTIBACTERIAL COMPOSITE BEADS BASED ON ALGINATE AND POLYHEXAMETHYLENE GUANIDINE HYDROCHLORIDE

Abstract

Polyhexamethylene guanidine hydrochloride (PHMG) is an antibacterial polymer compound of increasing
interest in water treatment, thanks to its strong activity and low toxicity. In this study, PHMG was
synthesized by hot-melt polycondensation and used to prepare antibacterial composite beads with sodium
alginate. The IR analysis results indicated that alginate/PHMG composite beads were successfully
synthesized, and there is a chemical interaction between alginate and PHMG. Therefore, the composite has
high stability, only 6.2% (wt.) of PHMG was released from the composite when it was soaked in water. The
synthesized PHMG has excellent antibacterial activity against gram-positive (+) and gram-negative (-)
bacteria and fungi, with a MIC value not exceeding 32 µg/mL. The water disinfection test showed that
alginate/PHMG composite beads can material 100% of ~ 103 CFU/mL E.coli have been killed, and that
composite can be reused 3 times.