WAVELENGTH SELECTION METHOD FOR UV-VIS SPECTROSCOPY TO SIMULTANEOUSLY QUANTIFY AMLODIPINE AND INDAPAMIDE IN TABLET FORM

Abstract

In this work, we employed the wavelength selection method to construct Partial Least Squares (PLS) models
for simultaneous quantification of Amlodipine (AML) and Indapamide (IND) in pharmaceuticals. Three
wavelength selection methods, including sensitivity ratio (SR), backward variable elimination (BVE), and
genetic algorithm (GA), were utilized. The results demonstrated that the SR method selected the highest
number of wavelengths, while the GA method selected the fewest. We also developed calibration models
including PLS, SR-PLS, BVE-PLS, and GA-PLS. The statistical values for both the calibration and
validation datasets were favorable. Evaluation based on recovery rate indicated that the recovery results
were within the range of 90-110% for both AML and IND. The repeatability also yielded satisfactory
outcomes, with RSD values < RSD calculated using the Horwitz function. The quantification results of AML
and IND fell within the range of 90-110% compared to the labeled content, adhering to the regulations of
the Vietnamese Pharmacopoeia V.