RESEARCH ON THE EFFECTS OF ENZYMES ON THE PROCESS OF GLUCOSE RECOVERY IN HYDROLYSIS OF PINE WOOD

Abstract

Acid pretreatment is also considered a s an optimal solution for the hydrolysis of lignocellulosic biomass by
removing lignin and part of hemicellulose in pine wood. However, there are still a number of organic
compounds derived from hemicellulose such as xylobiose, short-chain xylooligosaccharides and xylan that
have not been removed and hinder the hydrolysis ability of the cellulase enzyme. Studying the interaction
between enzymes and surfactants in the hydrolysis of cellulose and hemicellulose from pine wood pretreated
with formic acid helps enhance enzyme accessibility and promotes faster saccharification. In this study, the
enzymes Cellic Ctec 2 and Cellic Htec 2 together and the surfactant Triton X-100 were used as biocatalysts
for hydrolysis cellulose and xylan in pine wood. The surface response model (R2 = 0.99 and R2 – adj =
0.97) shows that the cellulase Cellic Ctec2 (p < 0.0001) has a relatively large influence on the ability to
hydrolyze cellulose into glucose. The results show that surfactants are involved in the hydrolysis of cellulose
and hemicellulose in pine wood after acid pretreatment, helping the enzyme operate stably for a long time,
increasing the ability of glucose recovery at the highest value of 295.2g/L.