STUDY ON THE DEVELOPMENT OF NEAR INFRARED SPECTROSCOPYFOR RAPID DETERMINATION OF SULFAGUANIDINE IN TABLETS

Abstract

    Near infrared spectroscopy (NIRS) method is now considered as a pharmaceuticalmanufacturing process. Quantitative NIRS give a fast acquisition, non-destructive,minimization of sample preparation step. In this study, content of sulfaguanidineintablets was deretemined by using NIR spectra in 3600-3000 cm- 1 combined withmultivariate linear resgression. Standars samples containing of different ratios ofsulfaguanidine to excipients were also prepared for setting up multivariate calibrationregression. A serries of 10test samples of formulation was also used to check theaccuracy of the models. The results showed that classical least square (CLS) andinverse least square (ILS) based on raw data of absorbance could not be sucessfullyapplied for quantitative analysis by NIRS. Principal component regression (PCR) hadbeen settup based on 3 PCs extrated from matrix of absorbance vs. wave number. TheNIR data for sulfaguanidine content was precise and similar to the lab referencemethod followed by Vietnamese Pharmacopoeia IV 2009.