ANALYSIS OF CARBOXY - DELTA 9 – THC IN URINE SAMPLES USING ULTRAPERFORMANCE LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (UPLC-MS/MS)

Abstract

The research reported the results of analysing Carboxy-delta 9-THC in urine samples of drivers using ultraperformance liquid chromatography coupled to a triple quadrupole mass spectrometer (UPLCMS/MS). Concentrations of Carboxy-delta 9-THC in urine samples were varied from 16ng/ml to 185ng/ml. The UPLC-MS/MS operated in multiple reaction monitoring (MRM) mode with positive ionization, analysis resulted in 10 minutes. Sample treatment using solid phase extraction with C18 gave recovery values more than 85%, the relative  repeatability standard deviation value was lower than 10%. The linear of Carboxy-delta 9-THC was tested in range from 10 to 500ng/ml in urine. The limit of detection and limit of quantification of the proceduce were 2,7 and 8,9ng/ml, respectively.