INVESTIGATION OF ANTIOXIDANT ACTIVITY OF METHANOLIC FRACTION EXTRACT FROM PINEAPPLE PEEL (ANANAS COMOSUS (L.) MERR.) AT TAC CAU, KIEN GIANG PROVINCE

Abstract

Pineapple is a fruit with high nutritional values. The aim was to investigate the antioxidant acitivities of
fractional methanol extract of pineapple peel. Using liquid-liquid extraction method with some solvents
range n-hexane, ethyl acetate, water-methanol would be evaluated of antioxidation capacities through
neutralization of free radicals DPPH, ATBS● and total antioxidant capacity (TAC) of these fractionated
extracts. Result, from methanol extracts of pineapple peel obtained 3 extract fractions, the highest
extraction efficiency was the fraction F3 (aqueous methanol extract, 48.23%), followed by the fraction
F2 (ethyl acetate extract, 29.30%) and the lowest with the fraction F1 (n-hexane extract, 12.66%). The
highest neutralization of DPPH and ATBS● was the ethyl acetate fraction with IC50 values as 220.46
µg/mL and 83.63 µg/mL, respectively. Similarly, the highest total antioxidant capacity (TAC) was also
ethyl acetate extract with Abs0.5 = 250.68 µg/mL. In short, the ability to neutralize free radicals DPPH,
ATBS●+, TAC of the ethyl acetate extract from Tac Cau pineapple peel extract was higher than nhexane extract and aqueous methanol extract.