Detection streptomycin using DNA aptamer and gold nanoparticle

Abstract

Streptomycin is one of common antibiotics belongs to aminoglycoside group that is widely used for treatment of gram-negative infectious diseases in fodder animals. However, incorrect and uncontrolled application of streptomycin could result in the presence of residues of this drug in foodstuffs and causes serious side effects on human. Therefore, it is very important to develop a rapid, simple and specific method to detect streptomycin in food products. In this study, a rapid method based on a streptomycin-specific aptamer and gold nanoparticle was developed for detection of streptomycin. In the presence of streptomycin, the competitive binding of the target and the DNA aptamer decreases the stability of gold nanoparticle in NaCl solution, triggers the aggregation, and exhibits visible color change of gold nanoparticle solution. This change can be seen by naked eye or UV-vis. Through UV-vis spectroscopic quantitative analysis, streptomycin can be detected at the concentration of 250nM. The presence of other aminoglycoside antibiotics shows neglectable disturbance. These results showed the established method may have enormous potential utility for practical streptomycin in food products in the future.